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Fiber Optic Reference Guide Ebook Rarl



A new textbook for training or studying for FOA CFOS/D and other certifications, and... An up-to-date reference on fiber optic design for the contractor, installer, designer, project manager, facilities manager and user.




Fiber Optic Reference Guide Ebook Rarl



The FOA has always tried to provide the world with sources of technically correct, unbiased information on fiber optics using both print and electronic media. The FOA Online Reference Guide on the FOA website is probably the largest and most used reference site on fiber optics on the Internet. The FOA Online Reference Guide provides a readily-accessed reference for FOA certifications for those schools using electronic texts. For those who prefer printed materials, we offer this book, a basic reference for fiber optics and a study guide for FOA certifications. Supplementary materials with even more depth on many subjects, of course, will be on the FOA website.


About the Author Jim Hayes, current FOA President, has over 40 years experience in the fiber optic communications industry as an technologist, entrepreneur and trainer. He is a co-founder of the FOA, author of 8 textbooks on fiber optics and cabling and a columnist for several trade magazines.


Because fiber-optical cable can carry any type of signal, and each connector type can be repurposed or custom built for a variety of different applications, the standards or proprietary manufacturer designs from which these connectors originated, or the standards organizations which now oversee their specifications, is the clearest method of categorization we could find.


Optical cannulas enable light to be delivered and collected from the brain. These probes are used in fiber photometry experiments. Due to their design, optical cannulas are only capable of collecting one signal or a population signal, providing no spatial resolution to image individual neurons. Depending on the length of the optical cannula, they can be used to collect a fiber photometry signal from shallow or deep brain regions. Another bonus is optical cannulas are the least invasive to be implanted in the brain because of their compact design, which leads to minimal tissue damage.


First, you need a coupling between the imaging probe, light source, and imaging device. This coupling enables the GECI to be illuminated in the brain through the imaging probe, and subsequently, the transmission of the emitting fluorescent signal is sent to the camera. Depending on the calcium imaging system design, this coupling may be achieved via an imaging fiber, optical fiber, or the system may be directly mounted onto the head of the animal, such as the miniscope.


Fiber photometry is an in vivo calcium imaging method that detects average fluorescence intensity changes. Thus, this method is used to measure population neural activity in a freely-behaving animal (Cui et al. 2014). With an implanted optical cannula coupled to an optical fiber, light is delivered and retrieved from the brain. The acquired signal is then collected by an externally positioned imaging device (photodetector, PMT, or camera).


The weight of the flexible imaging fiber and the head-mounted fixture is very low (as little as 0.7g). And, all the electronics are located off the head of the animal, compared to the miniscope. Thus, the length of experiments can be extended and extraneous factors (e.g., stress) can be reduced, allowing for more natural animal behavior to be observed with the optical fiberscope. To add to this, the recent implementation of an intricate rotary system enables better freely-behaving experiments with the optical fiberscope.


A vital benefit of the optical fiberscope is the unique flexible design. This system is scalable and reconfigurable, making it a generic calcium imaging and stimulation platform that can be adapted for different applications, unlike many other single-purpose systems. Two illumination paths allow researchers to attach multiple wide-field and/or targeted light sources with different wavelengths and to insert different optical filters (e.g., dichroics) suitable for different imaging and/or illumination needs. Also, this system is compatible with high-quality scientific cameras for capturing better quality images (e.g., with better signal-to-noise ratios and better linearity) for data analysis.


In comparison to both the miniscope and fiber photometry, the optical fiberscope can not only perform widefield optogenetics but also, by integrating a digital-mirror-device (DMD), be used to perform cellular-resolution optogenetics in vivo. The optical fiberscope is the only freely-behaving system capable of cellular-resolution calcium imaging and cellular-resolution optogenetics in a freely-behaving animal. Scientists can perform these experiments in the deep-brain or across a large area of the cortex.


Researchers can perform cellular-resolution calcium imaging and optogenetics in multiple regions simultaneously by using a split-fiber with the optical fiberscope. For example, one can stimulate multiple individual neurons or a selected group of neurons in one region and image individual cells in another, and this is not possible with fiber photometry or a miniscope.


The optical fiberscope is a powerful all-optical imaging and optogenetics system capable of both cellular-resolution optogenetics and cellular-resolution imaging in a single brain region or multiple brain regions simultaneously.


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